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The Dcp2 C terminus is required for Dcp2 instability. (A) Translation shutoff assays in HEK 293T cells transiently expressing the indicated Myc-tagged Dcp2 proteins. wt, wild-type Dcp2. The times above the lanes refer to the times (in hours) after translation shutoff using cycloheximide (Chx). Endogenous HuR was monitored as a control. (B) The same as panel A, but monitoring different Dcp2 mutant proteins, as indicated. Translation shutoff was performed with puromycin (Puro).
