FIG 7.

Immunoprecipitation of TAF10 and GATA1 in MEL cells. (A) GATA1 immunoprecipitation in MEL cells. Anti-GATA1 antibody (N6) was used for Western blot analysis. TAF10 and FOG1 are coimmunoprecipitated. (B) TAF10 immunoprecipitation in MEL cell nuclear extracts using the 6TA 2B11 antibody clone. IgG antibody was used as a control. GATA1 is detected in the IP fraction, confirming the MS results. Sup, supernatant. (C) TAF10 alone or a TAF8-TAF10 heterodimer was immunopurified using an anti-TAF10 antibody from SFf9 cell extracts and tested by Western blotting (WB) using the indicated antibodies. The GST-GATA1 protein or GST was also purified and tested by Coomassie brilliant blue (CBB) staining. (D) The purified proteins were combined as indicated above the gel and incubated, and after several washes the bead-bound proteins were denatured, resolved on an SDS-polyacrylamide gel, and analyzed by Western blotting using an anti-GST antibody. The antibody heavy chain (AbHc) and antibody light chain (AbLc) are indicated.