FIG 8.

Immunoprecipitation of TAF10 and GATA1 in MEL cells and mouse fetal liver at E12.5. (A) MEL cells and mouse fetal liver nuclear cell extracts were used to immunoprecipitate GATA1 endogenous protein or bio-GATA1 overexpressed in MEL cells. GATA1 (N6) IP or streptavidin pulldown (pd) coimmunoprecipitates GATA1/bio-GATA1 and TAF10 protein, as detected by Western blotting assays using M20 (GATA1) and 6TA 2B11 (TAF10) antibodies. BirA MEL cells were used as controls. (B) TAF10 (6TA 2B11) IP in MEL cells in which bio-GATA1 is overexpressed coimmunoprecipitate GATA1 in the reverse IP. HRP, horseradish peroxidase.