FIG 9.
ChIP assays of TAF10 and GATA1 in the GATA1 locus in hFL and hPB cells. A TAF10 antibody (monoclonal antibody 23TA 1H8) and a GATA1 antibody were used to immunoprecipitate the formaldehyde-cross-linked chromatin from hFL and hPB cells. (A) Primers for GATA1 binding sites of the human GATA1 locus, as indicated (the palindromic GATA1 binding site [BS] and GATA1 binding site at the kb −3 region relative to the TSS), were used to estimate the relative fold enrichment (RFE) of TAF10 and GATA1 binding by qRT-PCR. A CD71 antibody (isotype control) was used for the mock IP, and background enrichment was set equal to a value of 1. IE, exon I erythroid. (B) Enrichment for TAF10 and GATA1 at both binding sites of interest is shown in independent experiments for fetal liver and adult blood. (C) Overview of all ChIP experiments. See panel A for the positions of the primers.