TABLE 1.
Flow cytometry analysis of fetal livers
| Parameter | Embryonic day | Result for: |
P valuea | |
|---|---|---|---|---|
| Control mice | Taf10KOcEry mice | |||
| % live cells | E11.5 | 39.55 ± 7.51 | 38.20 ± 22.49 | NS |
| E12.5 | 63.39 ± 5.94 | 52.53 ± 4.16 | <0.005 | |
| E13.5 | 60.69 ± 3.21 | 6.55 ± 6.13 | <0.001 | |
| % c-Kit-positive cells | E11.5 | 22.45 ± 5.99 | 35.15 ± 4.60 | NS |
| E12.5 | 5.61 ± 0.98 | 11.47 ± 1.24 | <0.001 | |
| E13.5 | 3.85 ± 0.43 | 0.35 ± 0.40 | <0.001 | |
| % c-Kit- and CD71-positive cells | E11.5 | 37.78 ± 6.96 | 20.95 ± 15.20 | NS |
| E12.5 | 16.30 ± 2.84 | 26.43 ± 5.67 | <0.05 | |
| E13.5 | 8.03 ± 1.10 | 0.05 ± 0.03 | <0.001 | |
| % CD71-positive cells | E11.5 | 17.10 ± 5.85 | 7.79 ± 6.38 | NS |
| E12.5 | 69.48 ± 3.19 | 50.95 ± 6.16 | <0.01 | |
| E13.5 | 82.92 ± 2.09 | 14.50 ± 6.85 | <0.001 | |
| % CD71- and Ter119-positive cells | E11.5 | 29.00 ± 9.29 | 14.95 ± 10.54 | NS |
| E12.5 | 78.15 ± 1.99 | 55.93 ± 7.21 | <0.01 | |
| E13.5 | 89.99 ± 1.64 | 14.57 ± 8.79 | <0.001 | |
| c-Kit MFI | E11.5 | 11,121.63 ± 1,768.39 | 10,149.00 ± 2,604.98 | NS |
| E12.5 | 2,645.51 ± 126.30 | 3,331.04 ± 618.45 | NS | |
| E13.5 | 1,440.50 ± 331.33 | 221.98 ± 188.65 | <0.001 | |
| CD71 MFI | E11.5 | 41,095.75 ± 5,407.63 | 19,293.50 ± 12,581.55 | NS |
| E12.5 | 36,305.74 ± 2,721.79 | 31,207.59 ± 1,445.95 | <0.001 | |
| E13.5 | 43,745.10 ± 2,266.78 | 3,034.50 ± 1,274.59 | <0.001 | |
| Ter119 MFI | E11.5 | 1,312.75 ± 250.17 | 647.50 ± 7.78 | <0.001 |
| E12.5 | 3,175.30 ± 215.79 | 2,194.38 ± 323.44 | <0.005 | |
| E13.5 | 3,734.50 ± 339.00 | 359.75 ± 92.00 | <0.001 | |
a
Statistical analysis derived from the flow cytometry analysis of fetal livers of Taf10KOcEry and control embryos at E11.5, E12.5, and E13.5. At least 3 embryos were analyzed per genotype per stage. NS, no significant difference.