FIG 7.

CypA deficiency suppresses chondrogenic differentiation of primary mesenchymal limb bud cells. (A) H&E staining of sections of micromass pellets. (B) Alcian blue staining of sections of micromass pellets. (C and D) Immunohistochemical detection of Col2 (C) and Col10 (D) in micromass pellet cultures of mesenchymal limb bud cells from Ppia^+/+ and Ppia^−/− mice (E12.5). (A to D) Scale bar, 50 μm. (E) Western blot analyses of CypA and p65 expression levels in mesenchymal limb bud cells (E12.5) isolated from Ppia^+/+ and Ppia^−/− mice. GAPDH was used as a loading control. (F to I) Relative mRNA expression levels of Sox9 (F), Col2α1 (G), Col10α1 (H), and Runx2 (I) in 21-day-cultured micromass pellets. The data are shown as means and SD of three experiments. n = 4 in each group. *, P < 0.05 compared to chondrogenic differentiated limb bud cells from WT mice.