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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Methods Mol Biol. 2012;814:9–22. doi: 10.1007/978-1-61779-452-0_2

Fig. 3.

Fig. 3

Expansion of primary neural cells as purified astroglial precursors, adult human neural progenitor cells (AHNPs) from the adult human temporal cortex. (a) High passage (>60 population doublings) cells express astrocyte markers GFAP, S100β, and glutamine synthetase. Cytoskeletal nestin (expressed in dividing cells, inset) is also present. Cells counter-stained with DAPI. (b) Voltage-clamp membrane recordings of these cells reveal prominent Na+ and minimal K+ channel activity. (c) AHNPs derived from temporal cortex and hippocampus continue logarithmic expansion throughout culture. (d) Hippocampal and temporal cortex cells maintain a stable gliotypic morphology throughout culture. (e) Both hippocampal and temporal cortex cells maintain an equivalent stable doubling rate throughout culture. Scale bars: 50 μm (GFAP), 100 μm (additional images) (a), 150 μm (d) (adapted from Walton et al. (35)).