Table 1. Inhibitory effect of nitrate and (per)chlorate against sulfate-reducing bacteria, IC50 (mM) (95% confidance interval)a.
| NO3− | ClO4− | ClO3− | NO2− | ClO2− | |
|---|---|---|---|---|---|
| (A) Marine enrichment culture | |||||
| Growth | 46 (34–62) | 21 (14–31) | 44 (29–60) | 5.5 (3.3–9.3) | 2.8 (1–7.5) |
| Sulfide | 8.0 (7.0–9.0) | 2.3 (2.0–2.6) | 1.6 (1.4–1.8) | 0.12 (0.1–0.4) | 1.17 (0.8–1.7) |
| Desulfovibrionales | 6.1 (3.5–10) | 1.7 (1.3–2.2) | NM | NM | NM |
| dsrA copy # | 9.5 (7.8–12) | 1.9 (1.3–2.7) | NM | NM | NM |
| (B) Desulfovibrio alaskensis G20 | |||||
| Wild type | 51 (40–65) | 24 (20–32) | 6.3 (4.9–8) | 0.42 (0.32–0.56) | 4.7 (0.5–41) |
| tn5::rex (Rex mutant) | 250 (90–300) | 51 (37–70) | 25 (20–31) | 0.1 (0.09–1.2) | 9.6 (6.7–13) |
Abbreviations: ANOVA, analysis of variance; NM, not mentioned.
(A) IC50 against growth, sulfide production, dsrA copy number and Desulfovibrionales 16S abundance in marine enrichment cultures and (B) growth of Desulfovibrio alaskensis G20 and tn5::rex. Values for the 95% confidence intervals are shown in parentheses. Italicized values for sulfide or tn5::rex growth represent significant differences between growth and wild-type IC50 values respectively, ANOVA, P<0.05. Underlined values for sulfide or wild-type G20 growth represent significant differences between IC50 values relative to nitrate, ANOVA, P<0.05. IC50 values against Desulfovibrionales 16S abundance and dsrA copy number were not significantly different from IC50 values against sulfide production, ANOVA, P>0.05.