Table 2. Overall and pair-wise comparisons of Mud River bacteria community composition analyzed with perMANOVA using Bray–Curtis and GUniFrac distances for a priori groups and Bray–Curtis distance for post hoc groups.

Source	d.f.	SS	MS	F	R2	P
PerMANOVA for Mud River bacteria using Bray–Curtis distance, a priori
Overall
Site type	3	1.047	0.349	1.937	0.244	0.002
Residuals	18	3.245	0.18	0.756
Total	21	4.292	1
Contrasts
Mined vs unmined	1	0.573	0.573	3.18	0.134	0.001
Mainstem mined vs active valley fill	1	0.288	0.288	1.595	0.067	0.08
Active valley fill vs reclaimed valley fill	1	0.187	0.186	1.035	0.043	0.415
Residuals	18	3.245	0.18	0.756
Total	21	4.292	1
PerMANOVA for Mud River bacteria using GUniFrac distance, a priori
Overall
Site type	3	0.161	0.054	1.553	0.206	0.021
Residuals	18	0.621	0.034	0.794
Totals	21	0.781	1
Contrasts
Mined vs unmined	1	0.081	0.081	2.358	0.104	0.002
Mainstem mined vs active valley fill	1	0.054	0.054	1.576	0.07	0.061
Active valley fill vs reclaimed valley fill	1	0.025	0.025	0.724	0.032	0.748
Residuals	18	0.621	0.034	0.794
Total	21	0.781	1
PerMANOVA for Mud River bacteria using Bray–Curtis distance, post hoc
Overall
Site type	2	1.402	0.701	4.607	0.327	0.001
Residuals	19	2.89	0.152	0.673
Totals	21	4.292	1
Contrasts
AB vs unmined	1	0.573	0.573	3.768	0.134	0.001
Group A vs group B	1	0.828	0.828	5.445	0.193	0.001
Residuals	19	2.89	0.152	0.673
Total	21	4.292	1

Abbreviations: MS, mean sum of squares; perMANOVA, permutational multivariate analysis of variance; SS, sum of squares.