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. 2015 May 20;5:10488. doi: 10.1038/srep10488

Figure 6.

Figure 6

The increase of oxidative damage by inhibition of haem metabolism and the restoration by biliverdin and bilirubin. (a) The increase of cell damage with SA and restoration by biliverdin and bilirubin. HEK293T cells were incubated with 200 μM DEM and 20 μM menadione with or without 1 mM SA. At the beginning of incubation, 10 μM haemin, 40 μM CORM2, 1 μM biliverdin, or 0.1 μM bilirubin was also added to the culture medium. After 24 h of incubation, an MTT assay was carried out to evaluate cell damage. The data are expressed as the mean ± SD (n = 6 for each group). *, P < 0.01 and **, P < 0.05 vs. treatment with DEM plus SA. (b) Effect of Zn-protoporphyrin (Zn-PP), biliverdin, and bilirubin on oxidative damage. HEK293T cells were treated with chemicals as above, except for the use of 10 μM Zn-PP instead of the addition of SA. Cell damage was examined by MTT assay. Data are expressed as the mean ± SD (n = 5 for each group). *, P < 0.01.