Fig. 3.

HvTIP genes are differentially expressed in aleurone cells treated with GA and ABA. Barely aleurone layers were isolated from freshly imbibed seeds and incubated in medium containing 5 μM GA, 10 μM ABA, or no hormone, and total RNA was extracted at the indicated times to measure expression of each HvTIP member by qRT-PCR. Expression of each member of the HvTIP multigene family was normalized by the expression level of the internal control barley ubiquitin gene. The relative ratio between the transcript abundance of the HvTIP gene and ubiquitin gene was plotted on the y-axis, revealing the relative abundance of each HvTIP transcript. Error bars represent standard deviation.