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. 2014 Dec 3;66(5):1191–1203. doi: 10.1093/jxb/eru467

Fig. 6.

Fig. 6.

Expression of HvTIP3:1–GFP slows down the PSV coalescence process. (A) Diagram of the constructs used in the transient expression assay. The GFP vector used in the subcellular localization studies shown in Fig. 4A and the RNAi-TIP3;1 construct shown in Fig. 5A were used to examine the effect of HvTIP3;1 overexpression. (B) Transient expression assay for PSV coalescence. Barley aleurone protoplasts were co-transfected with empty vector/Ubi::GFP, HvTIP3;1–GFP/Ubi::GFP, or HvTIP3;1–GFP/Ubi::GFP plus RNAi-TIP3;1 and incubated in medium containing GA (10 μM) for 30h. After incubation, the protoplasts from each transfection were examined for the status of vacuolation using the five categories indicated in Fig. 4B. Bars indicate the number of protoplasts of each stage ± standard deviation after 30h of incubation with GA.