Fig. 7.

Cumulative expression of loci with AS between diploid and tetraploid watermelon quantified by RT-qPCR. Validation of RNA-seq data and semi-quantitative RT-PCR by RT-qPCR. Three technical and three biological replicates were performed for leaf (L), stem (S), and fruit (F) tissues from the diploid and tetraploid. Expression of the calibrator sample (diploid leaf; CLD1-L) was set to 1. The expression of each gene is the cumulative value of all transcripts per se, and unique primer sets were designed in common exon region. Data are means±standard deviation from three biological replicates.