Fig. 8.

Quantitative expression of selected splicing variants of a few spliced loci of watermelon quantified by RT-qPCR. Validation of semi-quantitative RT-PCR results with use of RT-qPCR for splicing analysis. Three technical and three biological replicates were used for leaf (L), stem (S), fruit (F) tissues from diploid and tetraploid. A few genes for certain tissues lacked a C _T value and are labelled as non-detectable (ND). The expression of any one of the samples was set to 1 for different genes. Primer sets were designed in exons with AS variants that were unique to certain transcripts. Data are means±standard deviation from three biological replicates.