Table 6.
RT-qPCR analysis of genes that differentially expressed across tissues in tetraploid compared with diploid watermelon
| Gene name | ICuGI Ontology/Function | Leaf | Stem | Fruit |
|---|---|---|---|---|
| Origin recognition complex subunit | Origin of replication/Recruitment of Cdc6 | 3.93±1.57 | 6.74±1.38 | –5.72±0.06 |
| GPI ethanolamine phosphate transferase 2 | Anchoring to the cell membrane | 1.98±0.11 | 1.46±0.21 | –585.34±0.00 |
| Serine-threonine protein kinase | Signal transduction | 2.74±0.32 | 86.42±7.34 | –1.40±0.31 |
| Inositol-3-phosphate synthase | Inositol biosynthesis | –49.61±0.00 | 1.43±0.60 | –6.36±0.05 |
| Indole-3-acetic acid-amido synthetase | Auxin homeostasis/disease resistance | 1.49±0.10 | –2.89±0.06 | –5.07±0.01 |
| Non-specific lipid-transfer protein 8 | Membrane transfer of phospholipids | 1.91±0.32 | –3.39±0.05 | –5.49±0.02 |
| Ferritin-2 | Iron storage and availability | –8.78±0.02 | 38.58±13.73 | –84.90±0.00 |
| Amine oxidase family protein | Deamination of polyamines required for cell growth | 28.81±1.91 | 9.21±5.56 | –1.21±0.25 |
| Phloem filament protein | Major protein composing phloem filaments | 4.56±1.18 | –2.63±0.04 | –3.46±0.14 |
| Cellulose synthase | Production of cellulose | 1.63±0.23 | 61.97±7.62 | –1.33±0.18 |
| MLP-like protein 28 | Defence response | 1.36±0.14 | –2.97±0.02 | –8.00±0.04 |
| Chloroplastic chlorophyll a-b-binding protein P4 | Light receptors for photosystems | 20.34±1.04 | 3.33±0.86 | –10.45±0.04 |
| Aquaporin PIP2.1 | Petal expansion/regulates water channels | 2.44±0.07 | 41.58±18.74 | 1.04±0.16 |
| ABC transporter A family member 8 | Regulates pump activity in membranes | 1.50±0.18 | –10.31±0.08 | –3.84±0.01 |
| Ethylene-responsive proteinase inhibitor 1 | Inhibition of protease activity | 1.14±0.20 | –2.67±0.17 | –4.60±0.03 |
| Cellulose synthase-like protein H1-like isoform 1 | Cellulose production | 2.64±0.10 | 4.05±0.44 | –21.89±0.01 |
| Ubiquitin ligase protein cop1 | Polyubiquitination/protein degradation | –6.98±0.01 | –6.53±0.02 | –15.20±0.01 |
| Dynamin-like protein C | Cell division/membrane trafficking | 113.66±23.43 | –5.15±0.02 | –8.48±0.01 |
| Serine carboxypeptidase | Protein cleavage/protein turnover | –878.63±0.00 | 77.87±32.89 | –17.47±0.01 |
Data are means±standard deviation relative to internal control 18S rRNA. Bold indicates upregulated in CLT1; italic indicates downregulated in CLT1.