Figure 3.

DNA methylation profiling at cadherin 22, precursor (CDH22) in prakriti and BMI. (A) Scatter plot illustrating positive, significant correlation (R = 0.22 and p ≤ 0.05) between the methylation values of CDH22 and BMI. (B) Distribution of log fold change differences of CDH22, 5′UTR associated CpG island. The differences with positive values in yellow and negative with blue indicate level of methylation variation between prakriti. The height of the individual bars represents the extent of methylation differences. Shaded in blue is a probe region showing a significant difference at p ≤ 0.2 between prakriti. The offset scale for log2 fold differences was set to −0.2 to 0.2. (C) Bisulfite genome sequence analysis. Methylation cut off >15% was considered to call methylated sites and are represented in black circles. The circles in white were considered as unmethylated with respect to the cut-off chosen. Rows indicate the sample and columns represent individual CpG site. (D) and (E), is the cumulative CpG site methylation variation in prakriti and BMI respectively. Each circle represents the overall methylation of amplicon for an individual. The methylation values in the groups is indicated with mean ± standard error and *refer to the significance (*for p ≤ 0.05 and **for p ≤ 0.01).