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. 2015 May 7;13:149. doi: 10.1186/s12967-015-0505-1

Figure 3.

Figure 3

Low relative recovery of miRNA. Mean normalized cycle quantification (Cq) for miRNAs showing a low relative recovery during microdialysis in vitro (1A, 1B, 1C) and in vivo (2). 1A: Cq values of miRNAs after in vitro microdialysis on total RNA from human brain. Dark grey column, 20 kDa membrane; grey column, 100 kDa membrane; light grey column, undialyzed reference sample. 1B: Cq values of miRNAs after in vitro microdialysis on CSF from a neurologically healthy patient. Dark grey column, 100 kDa membrane; light grey column, undialyzed reference sample. 1C: Mean Cq values of miRNAs after in vitro microdialysis on CSF obtained by lumbar puncture in a neurologically healthy patient. Dark grey column, 20 kDa membrane; light grey column, undialyzed reference sample. Error bars show SD (N of identical aliquots = 4). 2: Mean Cq values of miRNAs in in vivo microdialysate and CSF obtained from patients with subarachnoid hemorrhage. Dark grey column, in vivo microdialysate, 20 kDa membrane; light grey column, CSF. Error bars show SD (N of aliquots for each = 4). The four samples of each material was taken from two different patients with SAH on Day 3 and 9.