Table 1.

Schematic overview of pre-analytical and analytical setup

Sample number	Sample name	Source	Micro-dialysis	Catheter pore size	Perfusion fluid for microdialysis	qPCR – number of miRNA
1	RNA MD20	Human brain, total RNA	In vitro	20 kDa	CNS	377
2	RNA MD100	Human brain, total RNA	In vitro	100 kDa	HA 3.5%	377
3	RNA REF	Human brain, total RNA	None	NA	NA	377
4	H MD100	Healthy human, LP-CSF	In vitro	100 kDa	HA 3.5%	377
5	H REF	Healthy human, LP-CSF	None	NA	NA	377
6	SAH1 MD20A	SAH patient 1, EVD-CSF	In vitro	20 kDa	CNS	754
7	SAH1 MD20B	SAH patient 1, EVD-CSF	In vitro	20 kDa	CNS	754
8	SAH1 MD20C	SAH patient 1, EVD-CSF	In vitro	20 kDa	CNS	754
9	SAH1 MD20D	SAH patient 1, EVD-CSF	In vitro	20 kDa	CNS	754
10	SAH1 REFA	SAH patient 1, EVD-CSF	None	NA	NA	754
11	SAH1 REFB	SAH patient 1, EVD-CSF	None	NA	NA	754
12	SAH1 REFC	SAH patient 1, EVD-CSF	None	NA	NA	754
13	SAH1 REFD	SAH patient 1, EVD-CSF	None	NA	NA	754
14	SAH2D3 MD20	SAH patient 2, in vivo MD, day 3	In vivo	20 kDa	CNS	377
15	SAH3D3 MD20	SAH patient 3, in vivo MD, day 3	In vivo	20 kDa	CNS	377
16	SAH2D9 MD20	SAH patient 2, in vivo MD, day 9	In vivo	20 kDa	CNS	377
17	SAH3D9 MD20	SAH patient 3, in vivo MD, day 9	In vivo	20 kDa	CNS	377
18	SAH2D3 REF	SAH patient 2, EVD-CSF, day 3	None	NA	NA	377
19	SAH3D3 REF	SAH patient 3, EVD-CSF, day 3	None	NA	NA	377
20	SAH2D9 REF	SAH patient 2, EVD-CSF, day 9	None	NA	NA	377
21	SAH3D9 REF	SAH patient 3, EVD-CSF, day 9	None	NA	NA	377

qPCR, real-time polymerase chain reaction; miRNA, microRNA; MD, microdialysis; CNS, “Central nervous system perfusion fluid” (Mdialysis); HA, human albumin (CLS Behring); ref, reference; NA, not applied; LP-CSF, cerebrospinal fluid obtained by lumbar puncture; SAH, spontaneous aneurysmal subarachnoid hemorrhage; EVD-CSF = cerebrospinal fluid obtained from external ventricular drain.