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. 2015 May 20;15:123. doi: 10.1186/s12870-015-0500-y

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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OsTCTP expression patterns in transgenic rice plants. (A) Structure of constructs for rice transformation. The overexpression constructs (OsTCTPa and OsTCTPb) were developed under the control of the Ubi1 promoter and nopaline synthase (Nos) terminator cassette. (B) GUS staining of OsTCTPb overexpression transgenic rice plants. (C) Western blot analysis of OsTCTP protein from transgenic plants using an antibody against rice OsTCTP. Equal amounts (20 μg) were loaded to each lane and confirmed by coomassie brilliant blue (CBB) staining of Rubisco (bottom). The experiments were repeated for three times with similar results.