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. 2015 Jan 31;18(6):pyu096. doi: 10.1093/ijnp/pyu096

Figure 2.

Figure 2.

6-Chloro-7,8-dihydroxy-3-methyl-1-(3-methylphenyl)-2, 3,4, 5-tetrahydro-1H-3-benzazepine (SKF83959) treatment restores the decrease in hippocampal spine density and proliferation of hippocampal progenitor cells induced by defeat stress. (A) Representative confocal microscopic images showed the colocalization of NeuN (red) with doublecortin (DCX) (green) in the dentate gyrus (DG). The scale bar is 200 µm for representative images and 25 µm for enlarged images, respectively. (B) Chronic SKF83959 treatment significantly increased the number of DCX-stained cells in the DG of stressed animals (n=5). (C) Representative Western blotting of DCX showed that SKF83959 counteracted the deficiency of hippocampal DCX protein level caused by stress (n=5). (D) Representative photomicrograph of a Golgi-Cox stained pyramidal neuron of CA3 hippocampus. Scale bar=10 µm. (E) Summary data showed that the stress-induced decrease of spine density was restored by SKF83959 (n=6). Data are expressed as means ±SEM; # P < .05, ## P < .01 vs control; * P < .05, ** P < .01 vs defeated + vehicle group. Comparison was made by 2-way analysis of variance (ANOVA) followed by posthoc Bonferroni’s test.