Fig. 4.

The effect of LPA (10⁻⁵M) supplementation of oocyte maturation medium on apoptosis in COCs. Panel a depicts representative fluorescent images of bovine COCs used to TUNEL labeling: a negative control, b positive control, c, d control COCs, e, f COCs matured in the presence of LPA. White arrows indicate TUNEL stained apoptotic nuclei (green) in contrast to DAPI stained nuclei (blue). Bars = 100 μm. Panel b depicts quantitative analysis of LPA effect on apoptosis in COCs. The data are presented as a percentage of TUNEL positive apoptotic cells within all detected DAPI positive cells and expressed as mean ± SEM. Different letters indicate significant differences (P <0.05), as determined by Student’s t-test