Fig. 5.
The effect of LPA (10−5M) supplementation of oocyte maturation medium on cumulus expansion. Panel a depicts representative images of bovine COCs exhibiting three degrees of cumulus expansion: a grade 1 (non-expanded), b grade 2 (partially expanded) and c grade 3 (fully expanded). Original magnification × 50. Panel b depicts quantitative analysis of LPA effect on cumulus expansion. The values are presented as percentage of COCs and expressed as mean ± SEM. Capital letters indicate statistical significance (P > 0.05) between two treatments whilst different small letters indicate significant differences (P < 0.05) within each treatment, as determined by two-way ANOVA followed by the Tukey multiple comparison test