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. 2015 May 20;5:10405. doi: 10.1038/srep10405

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Cultured HPH were infected with Ad/EYFP-hCAR and treated with vehicle (0.1% DMSO), PB (1 mM) or each of the 10 selected agonists from qHTS assays at the indicated concentrations. After 24 h of treatment, hepatocytes were subjected to confocal microscopy for visualization of EYFP-hCAR localization. Approximately 100 EYFP-hCAR expressing cells from each treatment were counted according to the subcellular localization of hCAR in nucleus or cytoplasm.