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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Cell Rep. 2012 Dec 27;2(6):1579–1592. doi: 10.1016/j.celrep.2012.10.014

Figure 1. (A) Experimental design of the study.

Figure 1

Reprogramming was induced by expressing Oct4, Klf4, Sox2 and c-Myc. Cells were isolated at 3-day intervals by FACS sorting based on Thy1, SSEA-1 and GFP-Oct4 expression, followed by a quantitative proteomic analysis by pairwise comparison of two consecutive time-points. See also Fig. S1A. (B–F) Expression of transcription factors during reprogramming. (B) Expression profiles of the reprogramming factors. The average log2 expression change to day 0 +/− SEM for the transcription factors that were used to initiate reprogramming. C-Myc was not covered by the data, possibly because it is refractory to tryptic digestion. (C) Expression patterns of proteins in the core regulatory circuit associated with pluripotency. (D) Network of induced transcription factors that interact with the reprogramming factors. Increased expression early, transient or late is depicted in gray-scale. (E–F) Number of differentially expressed proteins that are targets of Oct4, Sox2, Klf4 or c-Myc (E) and other TFs (F). See also Figure S1.