Figure 5. (A) Functionally related proteins with opposing expression profile along reprogramming.

Curves show mean+/− SEM of log₂ expression change relative to day 0. (B–C) Principal component analysis reveals candidate proteins contributing particularly to a specific time-point. A PCA was applied to the approximated abundances (iBAQ) of each protein at each time-point. Proteins with probable high contribution to each time-point are highlighted in the biplot (B) and their temporal expression profiles shown in (C), with the same color-coding as in (B). (D) Proteins with a stage-specific expression pattern. Temporal expression profiles for examples of proteins with strong change (>fold 1.5) in at least one of the intermediate time-point comparisons, and only low change (<1.3-fold) or absence day 0 to day 3. Curves show mean +−/SEM of log₂ expression change to day 0.