Skip to main content
. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Dev Biol. 2014 Jul 1;393(2):209–226. doi: 10.1016/j.ydbio.2014.06.022

Figure 6. Lifeact-mEGFP labels actin filament bundles during S10B, but germline expression results in cortical actin breakdown.

Figure 6

(A-D”) Maximum projections of 3–5 confocal slices of fixed and stained S10B follicles taken at 20X magnification. (A-D) Merged images: DNA (DAPI) = cyan, F-actin (phalloidin) = white, Lifeact (anti-GFP) = magenta. (A’-D’) F-actin (phalloidin) = white. (A”-D”) Lifeact (anti-GFP) = white. (A-A”) Lifeact-mEGFP /+. (B-B”) c355 GAL4; Lifeact-mEGFP. (C-C”) Lifeact-mEGFP; nanos-VP16 GAL4. (D-D”) Strong germline GAL4 (either mat2MK, mat3, or oskar GAL4) driving Lifeact-mEGFP. Somatic expression of Lifeact-mEGFP does not alter nurse cell actin remodeling or follicle cell morphology during S10B (B-B” compared to A-A”). While Lifeact-mEGFP clearly labels cytoplasmic actin filament bundles within the nurse cells, both weak and strong germline expression of Lifeact-mEGFP results in cortical actin defects causing multi-nucleate nurse cells (C-D”, red circles). Scale bars = 50 μm.