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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: Dev Biol. 2014 Jul 1;393(2):209–226. doi: 10.1016/j.ydbio.2014.06.022

Figure 8. F-tractin-tdTom labels F-actin structures during S9 without causing severe actin defects.

Figure 8

(A-E”) Maximum projections of 3–5 confocal slices of fixed and stained S9 follicles taken at 20X magnification. (A-E) Merged images: DNA (DAPI) = cyan, F-actin (phalloidin) = white, F-tractin (anti-DsRed) = magenta. (A’-E’) F-actin (phalloidin) = white. (A”-E”) F-tractin (anti-DsRed)= white. (A-A”) F-tractin-tdTom/+. (B-B”) c355 GAL4; F-tractin-tdTom. (C-C”) F-tractin-tdTom; nanos-VP16 GAL4. (D-E”) Strong germline GAL4 (either mat2MK, mat3, or oskar GAL4) driving F-tractin-tdTom. Somatic expression of F-tractin-tdTom does not alter the nurse cell cytoskeleton or follicle cell morphology (B-B’’ compared to A-A’’). Similarly, follicles weakly expressing F-tractin-tdTom in the germline exhibit normal F-actin structures during S9 (C-C” compared to A-A”). While strong germline expression of F-tractin-tdTom does not cause morphologic defects (D-E”), it results in an increased frequency of early F-actin filaments in the posterior nurse cells (E-E”, orange box). Additionally, strong germline expression of F-tractin-tdTom results in the appearance of anti-DsRed positive, but phalloidin negative, puncta in the nurse cell cytoplasm (D”, E”). Scale bars = 50 μm.