Figure 5. M.tb induces NO production in human macrophages.

(A) Day 12 MDM monolayers on coverslips were incubated with M.tb, as described previously for 24 h, and then cells were incubated with DAF-FM diacetate, fixed, and examined by confocal microscopy with excitation/emission maxima of 495/515 nm (Olympus FluoView FV1000). Two representative micrographs (40×) of DAF-FM diacetate staining are shown for each test group of 3 independent experiments performed on duplicate coverslips. (B) Shown are cumulative data of the MFIs for each test group obtained from 3 independent experiments (mean ± sem; ***P < 0.0005).