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Outline of sample preparation and enrichment strategies. (A) Direct analysis of unfractionated tryptic digests. (B) Following tryptic digests but prior to LC injection, peptides are fractionated by isoelectric focusing (IEF). (C) Fractionation of intact proteins by gel filtration chromatography, followed by tryptic digest prior to LC injection (GF). (D) VHL capture probe-based enrichment of hydroxylated peptides.
