Sigma-Aldrich catalog no.	Stock (mg/mL)	Final in 1× NS2L (μg/mL)	Amount stock add to 100 mL neurobasal	Notes on stock solution
Aqueous stocks (store at 4°C)
L-Carnitine	C7518	2.0	2.0	5 mL	Resuspend all 10 mg in 5 mL dH20, add all of this
Ethanolamine	E0508	1.0	1.0	5 mL	Add 5 μL to 5 mL dH20, add all of this
D+-Galactose	G0625	15	15	5 mL	Weigh 75 mg and add to 5 mL dH20, add all of this
Putrescine	P5780	16.1	16.1	5 mL	Weigh 80 mg and add to 5 mL dH20, add all of this
Sodium selenite	S9133	0.01435	0.01435	5 mL	Resuspend all 1 mg in 70 mL dH20, add 5 mL of this
Ethanolic stocks (store under nitrogen at −70°C to minimize oxidation)
Corticosterone	C2505	2.0	0.02	50 μL
Linoleic acid	L1012	100	10	50 μL
Linolenic acid	L2376	100	1.0	50 μL
Lipoic acid (thioctic acid)		4.7	0.047	50 μL
Progesterone		0.63	0.0063	50 μL
Retinol acetate	R7882	10	0.1	50 μL
Retinal, all trans (vit. A)		10	0.1	50 μL
D,L-α-tocopherol (vit. E)	B5240	100	1.0	50 μL
D,L-α-tocopherol acetate	T3001	100	1.0	50 μL
Add fresh
Albumin, bovine	A4161	100	2500	12.5 g	Weigh out 12.5 g and add directly to neurobasal, first step in the process
Catalase	C40	16	2.5	80 mg	Weigh out 80 mg and add directly
Glutathione (reduced)	G6013	1.0	1.0	5 mg	Weigh out 5 mg and add directly
Insulin	I6634	4.0	4.0	5 mL	Weigh out 20 mg, dissolve in 5 mL of 0.1% acetic acid, add all of this
Superoxidase dismutase	S5395	2.5	2.5	12.5 mg	Calculate 12.5 mg from weight on three bottles and add directly (mix of new and old lots)
Transferrin	T5391	2.0	5.0	10 mg	Add all 10 mg directly (rinse bottle with the B27 mix)
T3 (triiodol-L-thyronine)	T6397	0.002	0.002	5 μL	Weigh out 10 mg, dissolve in 5 mL of 0.1 M NaOH, add all of thisa

^a

We typically omit T3 from the recipe to enable making media lacking T3 for some cell types. If T3 is omitted, it will need to be added to DRG Base Medium separately.