Skip to main content
. 2015 May 20;10(5):e0126437. doi: 10.1371/journal.pone.0126437

Fig 4. ELISA with sera from mice that survived aerosol challenge with B. mallei.

Fig 4

Serum samples were serially diluted and placed in duplicate wells of plates coated with GST-tagged BpaB (panel A) and CPS (panel B). Goat α-mouse antibodies (light and heavy chains) conjugated to Alkaline Phosphatase were used as secondary antibodies. The y-axis shows absorbance at a wavelength of 405 nm, which is indicative of antibodies binding to antigens coating the plates. The x-axis represents serial two-fold dilutions of sera. The results are expressed the mean absorbance (± standard error). Open diamonds show sera from mice that survived challenge with B. mallei ATCC 23344. Blue squares represent sera from control mice that were inoculated with 50 μL of PBS using the MicroSprayer. These serum samples were generated in the context of a previously published study [41].