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. 2015 Mar 4;12(1):199–209. doi: 10.3892/mmr.2015.3419

Figure 6.

Figure 6

Lymphocyte proliferation and specific lysis of JEV-infected P815 cells by DNA-vaccinated spleen cells stimulated with JEV. (A) T cell immunity generated by DNA vaccines was assayed. Splenocytes collected 3 weeks after the final immunization were examined for JEV-specific pro-liferative responses. (B) Specific lysis of JEV-infected P815 cells by DNA vaccine immunized spleen cells stimulated with JEV. Cytotoxic activities against JEV-infected P815 cells were measured at an E:T ratio of 10:1 using LDH activity as a marker of cell lysis. Data is expressed as the mean percentage ± standard deviation, n=4 of specific lysis. GM-CSF, granulo-cyte-macrophage colony-stimulating factor; JEV, Japanese encephalitis vaccine; pJME, premembrane and envelope proteins derived from JE virus; LDH, lactate dehydrogenase.