Table 1.
Molecular Characteristics of Colonizing ST131 Isolates From Long-Term Care Facility Residents in Olmsted County, Minnesota
| Category | Specific Characteristic (Definition)a | Prevalence Within ST131, No. of Isolates (% of 32) |
|---|---|---|
| O type | O25b (rfb variant; lipopolysaccharide synthesis) | 30 (94) |
| ST131 subclonal group | H30 (fluoroquinolone resistance-associated) | 32 (100) |
| H30Rx (ESBL-associated; subset of H30) | 9 (28) | |
| Adhesin | iha (adhesin-siderophore receptor) | 26 (81) |
| fimH (type 1 fimbriae adhesin) | 32 (100) | |
| Toxin | cnf1 (cytotoxic necrotizing factor) | 2 (6) |
| sat (secreted autotransporter toxin) | 26 (81) | |
| Siderophore receptor | fyuA (yersiniabactin receptor) | 32 (100) |
| iutA (aerobactin receptor) | 26 (81) | |
| Protectin | kpsM II (group 2 capsules) | 17 (53) |
| kfiC (K5 capsule) | 16 (50) | |
| K2/K100 (capsule variants) | 1 (3) | |
| traT (serum resistance-associated) | 25 (78) | |
| Other virulence genes | usp (uropathogenic-specific protein) | 32 (100) |
| ompT (outer membrane protease) | 32 (100) | |
| malX (pathogenicity island marker) | 30 (94) |
Abbreviations: ESBL, extended-spectrum-beta-lactamase; ST131, sequence type 131.
a Molecular characteristics not observed include the following (trait definition): afa/draBC (Dr-binding adhesins), afaE8 (variant afimbrial adhesin), astA (toxin of enteroaggregative Escherichia coli), cdtB (cytolethal distending toxin), bmaE (M fimbriae), clbB and clbN (colibactin polyketide synthesis), clpG (variant adhesin), cvaC (microcin V), F17 (variant adhesin), focG (F1C fimbriae adhesin), gafD (G fimbriae), H7 fliC (flagellar variant), hlyD (alpha hemolysin), hlyF (variant hemolysin), hra (heat-resistant agglutinin), ibeA (invasion of brain endothelium), ireA (siderophore receptor), iroN (salmochelin receptor), iss (increased serum survival), K1 and K15 (group 2 capsule variants), kpsMT III (group 3 capsules), papAH, papC, papEF, papG, and papG alleles I, II, and III (P fimbriae structural subunits, assembly, and tip adhesin variants), pic (protein associated with intestinal colonization), rfc (O4 lipopolysaccharide synthesis), sfa/focDE (S and F1C fimbriae), sfaS (S fimbriae adhesin), tsh (temperature-sensitive hemagglutinin), vat (vacuolating toxin).