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HCT116 cells exhibit changes in EMT markers and transcription factors, following Dox or Smad4-specific RNAi treatment. Expression of EMT markers and transcription factors was analyzed using (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting. β-actin expression was used as a reference gene for mRNA and protein expression. *P<0.05 vs. HCT116 cells and RNAi-NC cells. EMT, epithelial-mesenchymal transition; RNAi, RNA interference; NC, normal control.
