Table I.

Methylation-polymerase chain reaction conditions and primer sequences.

Primer	Sequences	Tm (°C)	Length (bp)
Methylation primers	Sense: 5′-TTCGAGGTGTTCGAGTAGTC-3′ Antisense: 5′-AACGATCTTC CGACA AAAAT-3′	65	310
Unmethylation primers	Sense: 5′-TTTTTTGAGGTGTTTGAGTAGTT-3′ Antisense: 5′-AACAAT CTTCCAACAAAAATACT-3′

Tm, annealing temperature; Length, the number of base pairs in the PCR products.