Figure 3.

Methylation-specific polymerase chain reaction analysis of the methylation status of GSTP1 and APC in BPH and PCa. (A) methylation status of the GSTP1 (U: 97 bp; M: 93 bp); (B) APC (U: 111 bp; M: 98 bp). The lanes are as follows: Marker; 1, H₂O; 2, BPH; 3, well-differentiated PCa; 4, moderately-differentiated PCa; and 5, poorly-differentiated PCa. U, unmethylated; M, methylated; BPH, benign prostatic hyperplasia; DNMT1, DNA (cytosine-5)-methyltransferase 1; GSTP1, glutathione S-transferase-P1; APC, adenomatous polyposis coli; PCa, prostate cancer.