Figure 7.

Activation of NF-κB assessed using EMSA in lung tissue obtained from the mice in each group. The EMSA gel is representative of five independent experiments. Semiquantitative analysis was performed by using UVP-gel densitometry. Arbitrary unit = (A_NC or A_AM or A_BAM or A_VAM or A_AAM/A_{unlabeled probes}) ×100%. Values are expressed as the mean ± standard deviation of five mice in each group. ^*P<0.05, as compared with the arbitrary units of activated NF-κB in lungs obtained from NC mice. ^#P<0.05, as compared with the arbitrary units of activated NF-κB in lungs obtained from AM mice. NC, normal control; AM, ovalbumin immunization-induced asthmatic mice; BAM: BML-111-treated AM; VAM, vehicle of BML-111-treated AM; AAM: anti-IL-1β antibody-treated AM; RAM, rabbit immunoglobulin G-treated AM; UP, unlabeled probes; NF-κB, nuclear factor-κB; EMSA, electrophoretic mobility shift assay.