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. 2015 Feb 17;12(1):45–54. doi: 10.3892/mmr.2015.3367

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Immunohistochemical analysis images (magnification, x200) demonstrating the expression of klotho in the kidneys of DM rats treated with (A) PBS (DM-PBS group), (B) GFP (DM-GFP group) and (C) mKL (DM-mKL group), as well as (D) SD rats treated with PBS (SD-PBS group). Scale bar=25 μm. The arrows indicate the protein expression of klotho in the renal tubule epithelial cells (brown staining). n=7 in each group. DM, diabetic; PBS, phosphate-buffered saline; GFP, green fluorescent protein; mKL, mouse klotho; SD, Sprague-Dawley.