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High resolution gradient gel 4-12% electrophoresis of the NAF proteins. Lanes 1, 8 and 13, molecular weight; lanes 2 and 5, blanks; lanes 3 and 7, bloody NAF; lanes 4 and 10, mixed NAF, lane 6, watery NAF; lane 9, citrine NAF; lanes 11 and 12, serous NAF. Arrows indicate the bands that were excised for subsequent proteomic analysis. NAF, nipple aspirate fluid.
