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. 2015 Mar 20;12(1):877–884. doi: 10.3892/mmr.2015.3524

Figure 4.

Figure 4

GTX promotes BMP-2-induced osteoblast differentiation in C2C12 cells. (A) BMP-2-induced ALP activity was enhanced by 1 μg/ml GTX treatment. (B–F) Gene expression levels of Col I, Ocn, Bsp, Runx2 and Osx were found to be enhanced by treatment with 1 μg/ml GTX + BMP-2, compared with those following treatment with BMP-2 alone. For the ALP activity assay, cells were incubated for 2 days and for the polymerase chain reaction assay, cells were incubated for 3 days. *P<0.05, **P<0.01, n>3. GTX, gliotoxin; BMP-2, bone morphogenetic protein-2; ALP, alkaline phosphatase; mRNA, messenger RNA.