Figure 3.

Liquid chip analysis of cytokines released by activated T lymphocytes. PBMCs were treated with PBS, 1 μg/ml CD28 Ab, CD3 Ab OKT3 or HER2/CD3 BsAb for 72 h. Release of the cytokines (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-2 was measured. ^*P<0.01, vs. other three groups. Ab, antibody; BsAb, bispecific antibody; CD, cluster of differentiation; HER, human epidermal growth factor receptor; PBMC, peripheral blood mononuclear cell; IFN, interferon; TNF, tumor necrosis factor; IL, interleukin; PBS, phosphate-buffered saline.