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. 2015 Mar 20;12(1):843–850. doi: 10.3892/mmr.2015.3522

Figure 4.

Figure 4

Effect of high glucose on the mRNA expression of Nampt, Sirt1, p53, P-gp and Top-IIα. SGC7901 cells were plated in 6-well plates and then incubated for 72 h in the presence of high glucose (4,500 and 9,000 mg/l) in order to examine Nampt, Sirt1, p53, P-gp and Top-IIα gene expression using reverse transcription quantitative polymerase chain reaction. Treatment of SGC7901 cells with high glucose resulted in increased (A) Nampt, (B) Sirt1, (C) p53, (D) P-gp and (E) decreased Top-IIα mRNA levels. Data are presented as the mean ± standard error of the mean (n=6). *P<0.05, **P<0.01 and ***P<0.001, v.s. the 1,000 mg/l group. Sirt1, silent information regulator 1; Nampt, nicotinamide phosphoribosyltransferase; P-gp, p-glycoprotein; topo-IIα, topoisomerase-IIα.