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. 2015 Mar 20;12(1):843–850. doi: 10.3892/mmr.2015.3522

Table II.

Gene sequences for quantitative polymerase chain reaction.

Human gene Forward primer Reverse primer
Nampt 5′-AAGAGACTGCTGGCATAGGA-3′ 5′-ACCACAGATACAGGCACTGA-3′
Sirt1 5′-TTGTACGACGAAGACGAC-3′ 5′-TAGAGCTTGCATGTGAGG-3′
p53 5′-TCAGTCTTCCTCCAACC-3′ 5′-GGAAAAGACTGAAGGGTG-3′
P-gp 5′-CATTCCTCCTGGAAATTCAACCT-3′ 5′-CTTCAAGATCCATTCCGACCTC-3′
Topo-IIα 5′-TGGAAACAGCCAGTAGAG-3′ 5′-ATCTTTGTCCAGGCTTTG-3′
β-actin 5′-CCTGGGCATGGAGTCCTGTG-3′ 5′-TCTTCATTGTGCTGGGTGCC-3′

Sirt1, silent information regulator 1; Nampt, nicotinamide phosphoribosyltransferase; GC, gastric cancer; P-gp, p-glycoprotein; topo-IIα, topoisomerase-IIα.