Table II.
Human gene | Forward primer | Reverse primer |
---|---|---|
Nampt | 5′-AAGAGACTGCTGGCATAGGA-3′ | 5′-ACCACAGATACAGGCACTGA-3′ |
Sirt1 | 5′-TTGTACGACGAAGACGAC-3′ | 5′-TAGAGCTTGCATGTGAGG-3′ |
p53 | 5′-TCAGTCTTCCTCCAACC-3′ | 5′-GGAAAAGACTGAAGGGTG-3′ |
P-gp | 5′-CATTCCTCCTGGAAATTCAACCT-3′ | 5′-CTTCAAGATCCATTCCGACCTC-3′ |
Topo-IIα | 5′-TGGAAACAGCCAGTAGAG-3′ | 5′-ATCTTTGTCCAGGCTTTG-3′ |
β-actin | 5′-CCTGGGCATGGAGTCCTGTG-3′ | 5′-TCTTCATTGTGCTGGGTGCC-3′ |
Sirt1, silent information regulator 1; Nampt, nicotinamide phosphoribosyltransferase; GC, gastric cancer; P-gp, p-glycoprotein; topo-IIα, topoisomerase-IIα.