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. 2015 Mar 13;12(1):1050–1058. doi: 10.3892/mmr.2015.3492

Figure 4.

Figure 4

NDRG1 affects the invasive potential of SHG-44 and U87 M glioma cells in vitro. (A and B) Cell invasion experiments demonstrated that transfection with RV-NDRG1 significantly inhibited the invasive capacity of U87 MG cells, and transfection with siNDRG1 promoted the invasive capacity of SHG-44 cells (stained with hematoxylin). (A) Magnification, ×40; (B) values are expressed as the mean ± standard deviation of experiments performed in triplicate. (C) Protein expression levels of N-cadherin, vimentin and E-cadherin in RV-NDRG1-transfected U87 MG cells and siNDRG1-transfected SHG-44 cells. β-actin was used as the internal control. (D) Single and merged images were taken to show immunofluorescence staining of N-cadherin (green) and vimentin (red), accompanied by nuclear staining (blue) with DAPI. NDRG1, N-myc downstream-regulated gene 1; si, small interfering RNA; RV, retrovirus; NC, negative control.