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. 2015 Mar 17;12(1):1023–1029. doi: 10.3892/mmr.2015.3501

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Copyright © 2015, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Construction of apoptin-modified MSCs. (A) Lentiviral vector pLV/Final-puro-EF1α-apoptin was identified by PCR. Lane 1/3, negative control; lane 2, amplification product of fragment of apoptin; lane 4, amplification product of fragment of EF1α promoter. (B) Expression of apoptin was confirmed by western blot analysis. Lane 1, negative control; lane 2, MSCs APOPTIN 1; lane 3, MSCs APOPTIN 2. (C) Morphology of MSCs hrGFP and MSCs APOPTIN. (D) Adipogenesis and osteogenesis differentiation of MSCs APOPTIN. Magnification, ×200. MSCs APOPTIN, mesenchymal stem cells expressing apoptin; PCR, polymerase chain reaction; hrGFP, humanized Renilla green fluorescence protein.