Fig. 5.

For the hydrogenated NHERF1∙^hFERM complex, the difference in D_eff(Q) between the rigid-body model and domain-motion models is very small, but is significantly increased in the deuterated complex. a Comparing the rigid-body calculation with the domain-motion calculation in the four-point model in the hydrogenated NHERF1∙^hFERM complex. NSE data from the NHERF1∙^hFERM (blue open squares), the four-point rigid-body model (black line), four-point model incorporating domain motion between PDZ1 and PDZ2 (red line), four-point model incorporating domain motion between PDZ1 and PDZ2 and finite size form factor of 20 Å radius for the FERM domain, PDZ1 and PDZ2 (blue line). D₀ at Q = 0 Å⁻¹ as measured from PFG NMR is shown in blue solid square. b Comparing the rigid-body calculation with the domain-motion calculation in the four-point model in the deuterated NHERF1∙^dFERM complex. NSE data from the NHERF1∙^dFERM (red open squares), the four-point rigid-body model (black line), four-point model incorporating domain motion between PDZ1 and PDZ2 (red line), four point model incorporating domain motion between PDZ1 and PDZ2 and finite size form factor of 20 Å radius for the FERM domain, PDZ1 and PDZ2 (blue line). D₀ at Q = 0 Å⁻¹ as measured from PFG NMR is shown in red solid square