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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Biomaterials. 2013 Nov 7;35(4):1302–1314. doi: 10.1016/j.biomaterials.2013.09.090

Fig. 1.

Fig. 1

PVBLG-8 allows effective transfection while induces appreciable cytotoxicity. (A) Reaction scheme of PVBLG-8. (B) CD spectrum of PVBLG-8 in DI water (0.1 mg/mL) at pH 7. (C) FITC-Tris uptake level in HeLa cells following co-incubation with PVBLG-8 for 2 h at 37 °C. (D) DNA condensation by PVBLG-8 at different PVBLG-8/DNA weight ratios as evaluated by the gel retardation assay. N represents naked DNA. (E) Particle size and zeta potential of PVBLG-8/DNA complexes. (F) Uptake level of PVBLG-8/YOYO-1-DNA complexes in HeLa cells following incubation at 37 °C for 4 h (n=3). (G) In vitro transfection efficiency of PVBLG-8/pEGFP complexes in HeLa cells at different weight ratios (n=3). (H) In vitro cytotoxicity of PVBLG-8/pEGFP complexes in HeLa cells at different weight ratios as determined by the MTT assay (n=3).