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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Biomaterials. 2013 Nov 7;35(4):1302–1314. doi: 10.1016/j.biomaterials.2013.09.090

Fig. 3.

Fig. 3

PVBLG-7 bears low cationic charge density than PVBLG-8 and thus exhibits weaker DNA condensation capability, lower membrane permeability, and lower cytotoxicity. (A) Reaction scheme of PVBLG-7. (B) CD spectrum of PVBLG-7 in DI water (0.1 mg/mL) at pH 7. (C) FITC-Tris uptake level of HeLa cells following co-incubation with PVBLG-7 for 2 h at 37 °C. (D) DNA condensation by PVBLG-7 at different weight ratios as evaluated by the gel retardation assay. N represents naked DNA. (E) Particle size and zeta potential of PVBLG-7/DNA complexes. (F) In vitro cytotoxicity of PVBLG-7 in HeLa cells as determined by the MTT assay (n=3). (G) In vitro transfection efficiency of PVBLG-7/pEGFP complexes in HeLa cells at different weight ratios (n=3).