Fig. 6.

Ternary complexes mediate efficient intracellular delivery of DNA via direct translocation and mannose receptor mediated endocytosis. (A) Uptake level of ternary complexes in HeLa cells in the presence of free mannose at various concentrations (n=3). (B) Uptake level of ternary complexes in HeLa cells at 4 °C or in the presence of various endocyt ic inhibitors. (C) FITC-Tris uptake level of HeLa cells following co-incubation with PVBLG-8 and PVBLG-7 at different weight ratios for 2 h at 37 °C (n=3). (D) CLSM images showing the cellular internalization and distribution of PVBLG-8/DNA binary complexes and PVBLG-8/PVBLG-7/DNA ternary complexes in HeLa cells following incubation at 37 °C for 4 h (bar = 20μm).