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. Author manuscript; available in PMC: 2015 May 20.
Published in final edited form as: Biomaterials. 2013 Nov 7;35(4):1302–1314. doi: 10.1016/j.biomaterials.2013.09.090

Fig. 7.

Fig. 7

Ternary complexes mediate higher transfection efficiencies than binary complexes. (A) Transfection efficiency of ternary complexes in HeLa cells at different PVBLG-8/PVBLG-7 weight ratios (n=3). PVBLG-8/DNA weight ratio was kept constant at 15, and for the PVBLG-7/DNA binary complexes, the optimal PVBLG-7/DNA weight ratio of 20 was used. (B) Representative flow cytometry spectra showing the transfection efficiencies of binary and ternary complexes in HeLa cells. (C) Fluorescent images of HeLa cells transfected with binary complexes (PVBLG-8/DNA weight ratio = 15) and ternary complexes (PVBLG-8/PVBLG-7/DNA weight ratio = 15:7:1) (bar= 100 μm). (D) Transfection efficiency of binary and ternary complexes in HeLa cells in the presence of free mannose at different concentrations (n=3).